Meios de cultivo e concentrações de BAP na embriocultura do pessegueiro "BRS Kampai"

Abstract

Early-ripening Peach cultivars present difficulties in the process of natural germination of seeds. In order to solve this problem, the culture of embryos in vitro can be considered an alternative in which the seeds find conditions to complete the germination and development in a satisfactory way. Different protocols and culture media have been tested to meet the nutritional needs of the embryo, but these still have problems and need to be optimized. The objective of the work was to test the addition of BAP to the culture medium, in order to increase the germination percentage of embryos and viable seedlings of 'BRS Kampai' peach, avoiding possible anomalies in the process. The experiment was conducted at the Tissue Culture Laboratory of the Federal Technological University of Paraná, Campus Pato Branco. The seeds submitted to embryonic culture come from plants maintained at UTFPR, in Pato Branco. The culture media tested were MS and WPM with five concentrations (0, 1, 2, 3 and 4 mg L-1) of 6-benzylaminopurine (BAP). Germination, in vitro development, stem length, survival, viable seedlings, and rosette formation were evaluated. Considering the conditions under which the experiment was conducted, it was concluded that the culture medium MS with the addition of 1 mg L-1 of BAP allowed a higher percentage of germination and viable seedlings for 'BRS Kampai'.