Estudo e síntese de biomaterial híbrido biocompatível TiO2 / colágeno para liberação de fármaco

Abstract

In this work, nanostructured TiO2 films were synthesized at 20 and 50 ° C in electrolytic medium containing simulated body fluid. After synthesis the films were used as matrix for the incorporation of drug and biomolecules. The films were incorporated with sodium cefazolin and to reduce the rate of release the collagen was used as the diffusional barrier. Drug delivery systems offer numerous advantages when compared to conventional methods, since the addition of a natural polymer such as collagen increases biocompatibility, as well as an efficient diffusional barrier membrane. SEM showed that nanotubular structures were formed in both conditions of synthesis, however, the film obtained at 50 ° C presented a more homogeneous morphology with no residual material on the surface. In the release assays it was observed that the materials obtained at 50 ° C showed a lower rate of drug release. In addition, type I collagen served as a diffusional barrier, decreasing the release rate of the drug per unit of time for both conditions of synthesis. For the in vitro osseointegration test, the XRD analysis confirms the growth of fluorapatite from the hydroxyapatite on the substrates obtained at both temperatures. In the hemolysis assay, all the biomaterials synthesized did not present severe hemolytic activity, that is, the erythrocytes remained intact in the solution, and the hemoglobin remained suspended indicating that they did not cause local hemorrhage. In general, biomaterials obtained at 50 ° C have been shown to be a good alternative for application as commercial implants, since they are biocompatible, assist in osseointegration and are efficient in the release of the drug for a prolonged period.