Atividade antimicrobiana de extratos de própolis produzidas por abelhas Apis mellifera selecionadas

Abstract

The propolis, produced by bees as a protector agent of the hive, has been used since antiquity for several civilizations. Among the propolis components, are the phenolic compounds, responsible for the antioxidant and antimicrobial propolis activities. The Federal Technological University of Paraná – campus Dois Vizinhos has been working in the development of better quality honey, produced by selected Apis mellifera bees. The objective of this study was to verify the antimicrobial activity of the extracts obtained in 2013 and 2015, following the 2³ factorial planning conducted for previews works using this propolis. After the extract obtainment, using the optimal extraction conditions, its antimicrobial activity was studied against Staphylococcus aureus and Salmonella bongori using the microdilution in 96-wells method for the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) determination. For the extracts that showed inhibition power, the MIC values varied between 0,31 and 2,5 mg.mL-1 for Staphylococcus aureus and between 1,25 and 5,0 mg.mL-1 for Salmonella bongori. When the MBC was determined for Staphylococcus aureus, the 2015 extracts were more effective than the 2013 extracts, obtaining MBC between 0,31 and 0,63 mg.mL-1. None of the extracts was efficient in the bactericidal effect of the Salmonella bongori. The results showed MIC values close to the ones found in the literature, being Staphylococcus aureus more sensitive to the extracts than Salmonella bongori.