Protozoários ciliados em ovinos suplementados com gordura protegida

Abstract

This study aims to quantify the ciliated protozoa in the rumen of sheep in relation to supplementation protected fat palm oil. The experiment was conducted at the Federal Technological University of Paraná, Campus Dois Vizinhos in animal metabolism UNEPE, from June to August 2014. Four male sheep with ruminal cannula were used with 50 kg body weight . These animals were kept in cages with water fountains and individual feeders fed 20% of Tifton 85 hay and 80% concentrate. The treatments were 0, 2, 4 and 6% added protected fat from palm oil. The experimental period was 80 days, with 14 days of adaptation and a day of collection of ruminal fluid, which was used for the quantification of protozoa and pH determination. The experimental design was Latin square (4x4), with four levels of inclusion of fat and four periods. The pH was determined with a digital pH meter, at 0, 2, 4, 6 and 8 hours after feeding the diet. The collection to quantify the protozoa was taken at zero and two hours. It was used adapted Counting chamber Sedgewick-Rafter, the collected samples were fixed in formalin 18.5% and then applied lugol solution (100 mL of distilled water five and ten grams of iodine g of potassium iodide), the mean scores were considered independent made of 100 fields performed in duplicate analyzed under a microscope with 10X magnification. The feed supplied to the animals were samples taken from days of collection and made pre-drying in forced ventilation oven at 55 ° C for 72 hours for further chemical analysis of dry matter, organic matter, crude protein and neutral detergent fiber. Data were subjected to analysis of variance and the effect of the inclusion level of fat was analyzed by regression. No significant difference was observed in total count of ciliate protozoa the rumen of sheep (P> 0.05) for inclusion levels of dietary fat protected (0, 2, 4 and 6%), however significant differences (P <0.05) between times 0 and 2 hours after feeding. Regarding the pH, there was significant difference (P <0.05) compared the diets provided and the time of collection and showed minimum level 6.1 with 0.18% inclusion of fat, 5.35 hours after power. The use of protected fat of palm oil up to 6% in the concentrate in the diet of sheep does not affect the population of rumen protozoa. The adjusted number of protozoa chamber allows to count according to the methodology recommended in the literature. There is a reduction Protozoa with decreasing pH and constant presence of oxygen.