Testes de concentrações de manipueira na produção de inóculo à base de Bacillus subtilis sobre o crescimento do trigo

Abstract

The concern with the impact that traditional agriculture entails in using agrochemicals for more immediate results has increased the demand for research in search of new alternatives to increase production without expanding agricultural frontiers. The present work aimed to evaluate the effectiveness of different concentrations of manipueira, an industrial residue with great potential for pollution, used as a substrate in the development of the bacterium Bacillus subtilis as a biological control agent and plant growth promoter for the purpose of low cost agricultural protection of Process, together with the benefit of providing an adequate destination for one of the major environmental liabilities of the flour and starch industries. Three media were formulated differing in the proportion of manipueira in its composition. Medium 2 (M2) with a concentration of 45% manipueira, 45% distilled water and 10% molasses stood out from the others reaching the concentration of approximately 6x1011 UFC per gram of the final inoculant produced. The germination rate of wheat was higher at all dilutions, the 0.5% dilution obtained the highest rate with 87.8% germinated seeds at 8 days after planting, differing in 43.4% of the control samples with 44.4% of the plants germinated in the same period of time. There was no significant difference between root and stem mass gain when using the inoculum at concentrations of 0.1% and 0.5% compared to the control, however, the high inoculant dosages affected both the root gain when of the stem as well as in the stature of the plant.