Screening de produtos naturais com potencialidade para tratamento de doenças ocasionadas por helicobacter pylori: um estudo in vitro e in silico

Abstract

Helicobacter pylori is a gram-negative bacterium that colonizes the gastric mucosa, which is associated with some pathogenic processes are inflammatory (chronic gastritis and peptic ulcer disease) or neoplastic (adenocarcinoma, and gastric lymphoma). The gastric infection with this bacterium is now considered the second most prevalent infections of man, is closely associated with a variety of clinical gastrointestinal disorders, this microorganism is the main etiological agent of more than 95% of chronic gastritis and duodenal ulcers. Characterized by the high rate of infections recorded, the constant search for potential drugs to eradicate this bacterium has performed essential. Thus, we evaluated the in vitro inhibitory activity of urease, chemical compositions and in silico studies of essential oils Rosmarinus officinalis (Rosemary), Illicium verum (Star anise), Cymbopogon citratus (Lemon grass), Cymbopogon winteranius (Citronella), Syzygium aromaticum (Clove), Eucalyptus globulus (Eucalyptus globulus), Citrus sinensis (Sweet orange), Citrus latifolia (Lima tahiti) and Citrus limon (Lemon). The results presented here indicate that essencial oils can be considered promising sources of bioactive substances inhibiting urease. Of the ten samples evaluated was determined that the essential oils of Rosmarinus officinalis, Cymbopogon citratus, Cymbopogon winteranius and Citrus sinensis urease inhibitors are promising. Studies in vitro and in silico converge to justify the inhibiting activity of urease inhibition by a mechanism based on the interaction of certain constituents of the essential oils in the active site of the enzyme.Thus, further studies are needed to see if these oils in vivo showed effect on H. pylori.