Desenvolvimento e validação de bioimpressora 3D e hidrogel de alginato/gelatina para produção de scaffolds 3D

Abstract

The high cost of bioprinters creates opportunities for research and projects to adapt 3D printers for application in biomaterials. In this work, we investigated the potential of using a low­cost 3D printer, customized with adaptation for commercial disposable syringes, which allowed for the controlled deposition of a customized hydrogel. Furthermore, a protocol for using the bioprinter for the production of hydrogel scaffolds was proposed. Therefore, it was necessary to adapt a low­cost 3D filament extrusion printer for printing with a plunger, a Graber I3 printer was adapted, after the adaptation, it was possible to simulate and evaluate the 3D bioprinting with the hydrogel formulation composed of alginate /gelatin. The conclusions were that the printer’s x, y, z movement system had uncertainties within the adequate standards, the ideal print speed was 5 mm/s and the extrusion multiplier was 0.10 mm3 with 100% cooling and a cold table. In relation to the hydrogel, the best result was for a formulation of 1.8% sodium alginate with 3% gelatin. The protocol for using the 3D bioprinter with the Alginate/gelatin hydrogel was evaluated and validated based on quality parameters.