Metodologia inicial para testes com bioimpressão 3D tendo como exemplo a impressão de neurônios motores e células musculares

Abstract

3D Bioprinting is an emerging field and has earned space among the prosthesis creating and drug development areas. Consisting of four major processes: creating or obtaining a 3D model in the form of CAD / CAM; formulation of bioinks; choice and execution of the printing process or method and post-print validation, 3D bioprinting presents itself as a vast area of multidisciplinary study. This study aims to develop a simplified methodology for initial steps on 3D Bioprinting, as well as describe the procedures involved. This methodology consists of the development of a protocol elaborated and applied to four tests created to analyze the printing capacity and viability of cells after printing. This work illustrates the use of this protocol applicating it to the case of the bioprinting process of neuromuscular junctions.