Estudo para determinação de herbicida 2,4-D em água através de CLAE-DAD

Abstract

One of the most important sectors in the Brazilian economy is agriculture and, therefore, the country becomes one of the largest consumers of pesticides in the world. In general, these are applied in pest control in order to increase productivity. However, if applied indiscriminately may cause serious damage to health of neighboring population beyond impacting various environmental matrices. Thus, several studies have been conducted to identify herbicides and evaluate their behavior in different environmental matrices. Many of these compounds are found in low concentrations, thus, the development of analytical techniques with high sensitivity is required to detect them. To this end, the chromatography is one of the main analytical techniques used for this purpose, detection of micropollutants. Therefore, this study aim to at determining and implementing a methodology for 2,4- D herbicide pattern analysis using liquid chromatography of high efficiency with diode array detector (HPLC-DAD). To this, they were evaluated two organic solvents (acetonitrile and methanol), commonly used in liquid chromatography for further testing and finally setting the optimum chromatographic conditions for detection of the herbicide. It was found that for the herbicide in question was the best condition found: methanol Elution: acidified water (70:30), 50mL injection volume, flow 1 mL min -1 , using the C18 column (ZORBAX Eclipse Plus - 4.6 x 250 mm, 5 um) and analyzed at a wavelength of 200, 230 and 280nm. By analyzing the figures of merit, the method was precise and linear, with a detection limit of 0.212 mg L-1 . It can be said that the herbicide is stable in acidified organic solvent and water when stored at 4 ° C temperatures.