Lacase imobilizada na biodegradação de efluente e corante têxtil

Abstract

Laccases are enzymes used in industries, can be used in the biodegradation of textile effluents due to their ability to biotransform several phenolic and amino aromatic compounds into non-toxic compounds. Presently, the processes used in effluent treatment use a variety of reagents and steps, which are often inefficient, do not completely degrade these compounds and require proper disposal. Tests were carried out with effluents generated in the Laundry and Dyeing Laboratories of the Federal Technological University of Paraná - Campus Apucarana, and laccase of Botryospheria rhodina MAMB-05, immobilized in Celite. By factorial design 22 (7 experiments) it was checked that the best immobilization was by experiment 1 (9ºC and 43 min), this obtained an enzymatic activity of 2.5 U/gsupport. With the immobilized enzyme, it was possible to treat the textile effluent in shaker at 150 rpm for 48 h. After 48 hours beyond visual analysis, UV-Vis spectroscopy was also performed. It is concluded from this study that the degradation of the dye by the laccase of Botryospheria rhodina MAMB- 05 was favorable in all experiments, with the CI Disperse Red 60 dye having 50 % discoloration in 5h and the dark effluent with 78 % discoloration in 24 h.