Relevância da Biologia Molecular - qPCR para o controle de qualidade em bactérias promotoras de crescimento vegetais e inoculantes vegetais: abordagem cienciométrica

Abstract

The soil microbiota plays a crucial role in maintaining soil health and fertility, as well as contributing to the healthy growth and development of plants. Plant inoculants contain Plant growth-promoting bacteria (PGPB), which can be used to address certain soil deficiencies, such as nitrogen and phosphate availability, and assist in the production of phytohormones, thereby improving productivity and reducing the use of chemical fertilizers. The use of molecular biology, particularly techniques like Quantitative Polymerase Chain Reaction (qPCR), offers advantages in the evaluation and quality control of these products, as they are faster and more specific than traditional methods used to ensure inoculant quality. Therefore, this work presents a scientometric analysis of the use of qPCR in the analysis of GPB and plant inoculants, aiming to map the impact and patterns of publications on these topics worldwide. The Web of Science (WoS) was used to select scientific articles, and CiteSpace was employed to create graphs corresponding to the patterns found. After filtering in WoS, 44 articles were identified, showing that qPCR is effective for quantifying specific microorganisms and differentiating beneficial species from contaminants. Additionally, the scientometric analysis highlighted the prominence of China, Brazil, and the United States in the studies conducted, indicating a growing global interest in the topic. Therefore, the increasing search for effective means to optimize plant production, along with qPCR as a valuable tool for inoculant evaluation, justifies the advancements in research conducted in recent years. The study underscores the relevance of current publications and can be updated over time to keep parameters related to molecular biology and inoculants up to date.