Emprego da microextração líquido-líquido dispersiva em fase reversa para preparo de amostra de fumaça líquida para determinação de cádmio e sódio

Abstract

The use of liquid smoke as an alternative to traditional smoke has become popular due to its greater practicality and control over industrial processes, especially in the preservation and flavoring of food, however, the formulation of oil-based liquid smoke raises concerns about possible contamination by inorganic compounds during its production process or application. In this study, a sample preparation method will be developed that involves reversed-phase dispersive liquid-liquid microextraction (RPDLLME). The RP-DLLME process comprises the following steps: sample heating, rapid addition of a mixture containing disperser and extractor, agitation to increase extraction efficiency, phase separation, aqueous phase collection, and determination of analytes by flame atomic absorption spectrometry (FAAS). After the RP-DLLME step, the presence of cadmium and sodium in the oil-based liquid smoke samples was analyzed. Tests were conducted to determine the best condition and volumeanalyte of the solvente n-propranol as disperser used 800µL (v.v-1), and extractor, extractor HNO3, 200µL (v.v-1), concentration 0,5 mol.L-1 HNO3 and volume ratio between dispersant and extractor was 1000µL(v.v-1), water bath heating temperatura 75°C for 15 minutes manual stiring time, agitation time 15 seconds and centrifugation 10 minutes at 5600 rpm. After the optimization of the conditions, the proposed method was applied, to determine cadmium and sodium in parts per million to analyze the analytes in a liquid smoke sample used in pasta.