1,25-Dihidroxicolecalciferol Glicosídeo no desempenho e na expressão gênica em progênies de matrizes pesadas

Abstract

This study aimed to evaluate productivity, egg weight and use of egg production from breeders and the performance and gene expression of calbindin-D28K, interleukin-10 and interleukin-1β in 21-day-old broiler chickens, generated by hens of different ages (30, 46 and 62 weeks) supplemented or not with 1,25(OH)2D3-. The design used was completely randomized in a 2x3 factorial scheme, in which 2 vitD supplements were tested (with and without supplementation) and 3 different ages of sows (30, 46 and 62 weeks of age). For the initial part, 8,000 sows of the AP95 lineage at 21 weeks of age were used, distributed in two treatments, the sows that received a diet supplemented with 100 g ton-1 of 1.25(OH)2D3-G, and dams that according to shed received a diet without supplementation. At 30, 46 and 62 weeks of age, part of the eggs from each house were separated for analysis of productivity, egg weight and use. The remaining eggs were incubated and transferred to hatchers. After birth, 1152 male broiler chicks were divided into three performance tests. Progeny tests were carried out using these broiler chicks from breeders aged 30 weeks, 46 weeks of age and 62 weeks of age. The progenies by sow age were distributed into two treatments, referring to the sows that received or did not receive supplementation with 100 g per ton of 1.25(OH)2D3-G in the diet from 21 weeks of age. The 30-week-old breeders had higher egg productivity, but lighter eggs compared to the older breeders, especially the 62-weekold breeders. The progenies born from dams with 46 and 62 weeks of age and supplemented with 1,25(OH)2D3-G, presented a higher birth weight, when compared to the progenies from dams with 30 weeks of age, as well as a greater consumption of feed, weight gain and lower feed conversion at 21 days. An increase in gene expression was observed in the progenies of matrices supplemented with 1,25(OH)2D3-G for the genes CALB-D28K, IL-10 and IL-1β compared to the progênies of matrices without supplementation. Considering the age of the sows, 1,25(OH)2D3-G supplementation in sows over 62 weeks of age resulted in increased gene expression of CALB-D28K, IL-10 and IL-1β in their progenies.