Atividade in vitro de óleos essenciais de plantas medicinais em carrapato bovino

Abstract

The objective of the present study was to evaluate the in vitro efficacy of the essential oils of Cymbopogon winterianus, Tagetes patula, Artemisia absinthium and Lippia alba on Boophilus microplus teleogines. At the beginning of 2019 (February) rural properties were selected that working with dairy cattle in the western region of Paraná to be used as source of R. (B) microplus. At the same time, the plants were collected to obtain the essential oils in the Garden of Medicinal Plants of the Biological Refuge of Santa Helena. The ticks were collected manually and sent to the UTFPR - Campus Santa Helena Zoology laboratory for conducting the bioassay. In order to perform the bioassay, the teleogynes were submitted to the treatments using the immersion method. As a negative control, acetone was used whereas the positive control consisted of amitraz®. The experimental design was completely randomized with 6 treatments and 30 replicates, each replicate consisting of a telegyn. After application of the treatments, the ticks were individually fixed in petri dishes with double-sided tape and conditioned in BOD with a constant temperature of 28ºC and relative humidity of 70-80%. Seven days after the treatments were applied, the number of dead ticks and eggs weighing were evaluated. The essential oils evaluated in the present study did not cause an increase in mortality and reduction in egg weight of R. (B) microplus.