Multirresistência de Enterococcus sp isolados do Lago Igapó na cidade de Londrina - PR

Abstract

The analysis of bacterial indicators in water is a very effective and sensitive method for pollution detection. Bacteria of the genus Enterococcus are regarded as indicators of fecal contamination, as part of the human body and the intestinal flora of some warm-blooded animals. This study aimed to isolate and verify the multidrug resistance of Enterococcus sp from the Lagos Igapó I, II and III in the city of Londrina-PR. The collection points were an area used for recreation and other more remote area that is not used for recreation. For the isolation of Enterococcus sp. It was used the membrane filter technique, where the membranes were transferred to Petri dishes containing esculin agar azide kanamycin and incubated at 37 ° C for 24 hours. They randomly selected 30 colonies of Enterococcus and to confirm the genus and species were held phenotypic analysis (Gram Positive, catalase, growth in 6.5% NaCl, pH 9.6, and temperatures of 10 and 45ºC) and genotypic employing the technique Polymerase Chain Reaction. To observe the susceptibility to antimicrobial agents was employed to disk diffusion technique as established by the CLSI. As a result we obtained that 100% of the isolates belonged to the genus Enterococcus. Of the 30 isolates 47% were identified as E. faecium, E. gallinarum 26.7%, 6.7% E. casseliflavus / E. flavences and Enterococcus spp. 13.33%. The susceptibility to antimicrobial 100% of the species were sensitive to Gentamicin, Norfloxacin, Chloramphenicol, Streptomycin and Ciprofloxacin. Already strength results obtained that: 96.7% of the isolates were resistant to vancomycin, penicillin; 10% tetracycline and 20% Amipicilina. To observe the susceptibility to heavy metals was used the technique of inhibitory concentrations of metals in gradient plate. As a result we obtained 100% of the strains were resistant to copper and chubo and 88% were resistant to zinc. Several isolates tested were resistant to more than two antibiotics, characterizing multidrug resistance. From the results presented in this study we can conclude that there Enterococcus sp in Igapó Lake that is used by the public as a recreational pond and that these show resistance to heavy metals and a wide range of antimicrobial routinely used in the treatment of enterococcal infections complicating therapy and harming not only the health of people who use the lake but also the aquatic life present in that environment.