Poder microbicida de antissépticos utilizados no manejo de ordenha frente à Escherichia coli e Staphylococcus aureus

Abstract

One of the diseases that most affects the dairy herd is mastitis and it can lead to a decrease in milk production causing economic losses to the producer. In order to control this disease, it is important the implementation of hygienic-sanitary measures such as the use of pre and post-milking products. Therefore, this work aimed to evaluate the in vitro effect of commercial antiseptic products used before and after milking of dairy cows by immersion of the teats. For the experiment it will be used one pre-milking product and two post-milking products. The antimicrobial activity against the microorganisms Escherichia coli and Staphylococcus aureus were analyzed. The experiments was carried out using the micro-and macrodilution broth techniques in the Microbiology laboratory of the Federal Technological University of Paraná, Campus Dois Vizinhos. The technique of microdilution broth seeks to determine the minimum inhibitory concentration and the minimum bactericidal concentration while the macrodilution technique has the purpose of verifying if the products prevent and eliminate contaminants that causes mastitis in different times of exhibition. In the microdilution test, E. coli presented resistance, whereas S. aureus showed to be sensitive to all the products tested, allowing to verify that they presented MIC and even CBM diluted. In macrodilution tests, the E. coli microorganism also proved to be resistant to all evaluated exposure times, S. aureus was sensitive to all products, demonstrating that the products are effective in inhibiting this microorganism. The antiseptics act in order to prevent the action of the microorganisms that cause mastitis, so, to evaluate the efficacy of these products is of paramount importance.