Análise da diversidade genética da espécie Dendrobium nobile Lindl. (Orchidaceae) no estado do Paraná

Abstract

This search has been automatically translated for Dendrobium nobile Lindl. in four regions of Paraná. The experiment was carried out in the laboratory of Molecular Biology of the Universidade Tecnológica Federal do Paraná - Dois Vizinhos campus, where 40 specimens of D. nobile were used, the two cities were Maringá and Cianorte, Dois Vizinhos and Mangueirinha, Guaramiranga and Ponta Grossa, Serranópolis and Matelândia, being five orchids for each city, after collection the specimens were taken to the laboratory for DNA extraction with the Wizard Kit from Promega, followed by PCR and genotyping of SSR primers. Microsatellites were analyzed by number of alleles per locus, allele frequency and polymorphism content (PIC). The obtained results show a greater genetic diversity among the populations than within the population, it was also possible to perceive that a number of allele sharing. The PIC for the primers was ER30 (0.3725); ER45 (0.3744) and ER59 (0.3047). With the present work we can see the efficiency of SSR markers for D. nobile. However, it is necessary to use a larger number of primers in order for the result to be more concrete,.