Avaliação de crescimento e determinação da concentração letal média (CL50) da espécie botânica Ruta graveolens L. e do fármaco nitazoxanida em vetor das principais arboviroses no Brasil, Aedes aegypti

Abstract

Aedes (Stegomyia) aegypti (Linnaeus, 1762) has a worldwide distribution, especially in the tropical and subtropical regions, and is a major public health problem, since it is the main vector of numerous arboviruses. Dengue is one of the most important mosquito-borne viral diseases due to the significant geographical spread of the virus and its vector in previously unaffected areas. Therefore, the search for new forms of mosquito control is essential, since Ae. aegypti has acquired resistance to chemicalsynthetic insecticides due to its excessive use. The present study aims to present a strategy to accelerate the life cycle of Ae. aegypti with addition of food and inputs such as coconut water, milk and milk casein obtained by isoelectric precipitation. The objective of this study was to determine the average lethal concentration (LC50) of the botanical species Ruta graveolens L. and the drug nitazoxanide in order to evaluate the toxicity and lethality in the larval phase of Ae. aegypti, caused by the action of their respective ethanol extracts. Cytotoxicity bioassays were performed using the standard procedure of the World Health Organization (WHO), with Ae. aegypti in the 3rd instar. As a result it was possible to successfully accelerate Ae. aegypti if using dairy food sources and coconut water. Regarding the determination of LC50, the study using third instar larvae of the mosquito showed that after 24 h of exposure, the LC50 of nitazoxanide was 10 mg.mL-1 and Ruta graveolens was 30 mg.L-1.