Hidrogel de quitosana e pectina aplicados para liberação controlada e matriz scaffold

Abstract

Hydrogels are formed by natural or synthetic materials and they have high waterbody fluids absorption capacities. Polysaccharides may be formed by means of chemical or physical cross-links, allowing application in a broad spectrum of scientific fields. Chitosan (a cationic polysaccharide) and pectin (an anionic polysaccharide) may be combined to promote formation of a hydrogel with application in the medical field. Thus, this work aimed the obtaintion of a hydrogel composed by both polysaccharides. In order it was assessed its biocompatibility, its potential to promote cell growth and also the its potential to act as a device to release gallic acid under different pH conditions. Analyses indicated that hydrogel is formed owing to intermolecular interactions (especially interaction via hydrogen bonding), between the polysaccharide chains. The surface of the hydrogel had large porous (364 ± 122 μm), allowing its application as matrix support to promote cell growth of preosteoblasts MCT3-E1 cells on rats, since the extracellular matrix structure seems with the hydrogel structure. Analysis with MCT3-E1 cells showed that hydrogel had capacity to proliferate the growth of MCT3-E1 cells to 125% at 10 days of incubation, compared to negative control (100%). The hydrogel also showed excellent biocompatibility, especially on healthy cells of African green monkey (Vero cells). However, controlled release studies of gallic acid were not satisfactory, due to the high porosity of the material.