Obtenção de esporos de Bacillus thuringiensis utilizando milhocina como substrato

Abstract

The present study consists in optimizing a fermentation process directed to obtain Bacillus thuringiensis (Bti) spores aiming its commercialization as bioinsecticide, through the fermentation of available substrates in the Center-North Region of Paraná State. Milletine has potential as an alternative substrate source for fermentation processes to obtain Bti spores. Experiments were conducted to evaluate the latent of this residue in conjunction with other reputable nitrogen and carbon sources, such as yeast extract, ammonium sulfate and glucose. In addition to testing the individual ingredients for the standard Tripticase Glucose Yeast (TGY) culture medium, a central composite design was performed in which alternative nitrogen sources in conjunction with glucose concentrations were analyzed for their behavior and reactivity expected to be noticeable during the fermentation process. It is technically possible to optimize the fermentation process to obtain Bti spores from fermentation using millocin. The optimization of the experiment resulted in a medium with the following concentration ranges of carbon and nitrogen sources: glucose 1g / L; yeast extract 2g / L; 40 mg / L millocin and 5 g / L ammonium sulfate. The millocin residue has great technical potential to be used as a source of nitrogen and carbon in fermentation processes.