Otimização de metodologia para identificação e quantificação de erucamida utilizando CLAE-DAD

Abstract

Erucamide is used as a glide additive in polymeric matrices due to its ability to migrate to the surface and modify the properties of the materials. In this work, a High-Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD) application is proposed for the identification and quantification of erucamide. The main objectives of the project are to develop a robust and sensitive method for the identification and quantification of erucamide. Acetonitrile and methanol were evaluated as mobile phases, and the intra- and interdia precision, linearity, limits of detection and quantification, and linearity settings were assessed. In previous studies, erucamide was incorporated into a nanocomposite formed by zinc oxide nanoparticles supported on montmorillonite, with the aim of favoring the surface migration of the active material when applied to linear low-density polyethylene (LLDPE) films, enhancing its antimicrobial action. The tests performed demonstrated that the retention time accuracy is within the validation criteria recommended by ANVISA (Brazilian Health Regulatory Agency). Ideal values for relative standard vision are up to 5%, considered indicative of excellent repeatability. Values between 5% and 10% are generally accepted. The inter-daily and intra-daily retention time accuracy values were less than 6%. However, in the case of the inter-daily accuracy calculated for the area, the values presented are outside the ideal or acceptable limit, with the lowest value being 15.7%. For the intra-daily accuracy values for the area, concentrations below 50 mg/L had accuracy values greater than 15%, and concentrations above 50 mg/L reached values within the acceptable range. The calculation of the detection and quantification limits using the signal-to-noise ratio method yielded high results of 19.8 mg/L and 59.3 mg/L, respectively. These results support the predictions of the proposed method for identifying and quantifying erucamide when found in high concentrations.