Protocolo para cultura de tecido vegetal

Abstract

In vitro cultivation is one of the techniques that emerged and has been improving in the last century. Due to the demand for high-performance genetic material, for example, we can mention the large production of plant seedlings that have great commercial power. Thus, this work establishes a protocol for in vitro cultivation of Vegetables at UTFPR Ponta Grossa. For that, the procedures adopted were the standards for carrying out the in vitro cultivation of Nicotiana tabacum, which is a herbaceous and perennial plant of tropical climate that has commercial value, and the seedlings of red pitaya created in an aseptic environment, we analyzed the best practical methods to being carried out by the researcher in each phase of the culture analyzed, the phases that were studied are mainly the phases of formulation and concentration of macronutrients in the culture medium, the preparation procedures to avoid contamination as much as possible and the viability of the plant over time.