Kit didático para demonstração da atividade enzimática da catalase

Abstract

Enzymes are proteins that catalyze chemical reactions in biological systems and are indispensable for the functioning of cells in all organisms. Thus, due to their role in understanding cellular mechanisms, in an educational context, the content on enzymatic activity is extremely relevant to be discussed in the discipline of Biology. In addition to being important for the human body, the performance of enzymes can also be seen in everyday life, such as the enzymatic activity of catalase, because when this enzyme comes into contact with hydrogen peroxide, it is possible to visually observe the formation of bubbles, with the release of water and oxygen. Despite the relevance of this subject for teaching, there are many challenges faced by teachers. In view of the above, the objective of the present work was to develop a didactic kit to demonstrate the enzymatic activity of catalase, to be used together with a pedagogical guide in e-book format. For the elaboration of the protein extract of the didactic kit, at first, experimental tests were carried out in order to verify the definition of the recipe, the validity and the final demonstration of the catalytic action of catalase. Subsequently, the kit was structured, consisting of test tubes, Pasteur pipettes, glass containers, pH measuring tapes, beaker, tube rack and support for containers, in addition to an organizing box to store all the elements. Also, an e-book was prepared that could serve as a guide for teachers, for future application in educational practices. In addition, an account was created on the Instagram application to disseminate the didactic material. In short, it is concluded that the development of the didactic kit to demonstrate the enzymatic activity of catalase and the e-book represent a valuable contribution to the teaching of enzymes.