Determinação do acúmulo de lipídeos em levedura residual da indústria cervejeira aplicando a técnica de Sudan Black

Abstract

The present study aims to investigate the capacity of Saccharomyces cerevisiae yeast, a byproduct of the brewing industry, to accumulate lipids, emphasizing the potential of harnessing this ability for the production of renewable energy, such as biodiesel, and for the reuse of residual products. The use of residual yeast focuses on leveraging the biotechnological potential of microbial oils produced by oleaginous cells, known as Single Cell Oil (SCO). Lipid production by these cells requires cultivation with excess carbon and nitrogen limitation. To assess lipid production, the Sudan Black staining technique was employed, using the Pearse method to monitor lipid accumulation in the yeast. Using the YEPG culture medium with yeast extract and an incubation time exceeding 300 hours, significant lipid production was observed. It is concluded that S. cerevisiae is capable of accumulating lipids and can be used in future studies to optimize lipid production, with potential applications in biotechnology.