Análise comparativa de proteínas totais teciduais e morfometria muscular em camundongos submetidos à suplementação com diferentes fontes proteicas

Abstract

Encouraged by environmental and economic reasons, the present study tested a mycoprotein, developed by the Xenobiotics Research Unit at PUCPR, using the microorganism Rhizopus oligosporus, through production bioprocesses for fungal biomass. Such biomass is already used as a base for tempeh, but is not produced alone for the production of fungal protein as a base for dietary formulations. 30 mice were evaluated, separated into three groups, with different protein sources (fungal, animal and vegetable as control). The initial masses were measured and compared with the masses at the end of the experimental period. From the 25th day of diet until the moment of euthanasia, feces were collected and stored at -5 ºC. After 30 days of controlled diet, the animals were euthanized with thiopental, and a biopsy of fragments of right and left soleus, liver and kidney tissues was performed. Nitrogen excretion was quantified in feces. In kidneys and livers, nitrogen concentrations were evaluated. The results obtained greater body mass gain in animals treated with an animal protein diet, while there was no difference in body masses gained between the fungal protein and control groups. The amount of nitrogen in the liver was higher in animals on the fungal diet, while in rinses and nitrogen concentrations were equal. The morphometric data indicated that in sedentary mice, there were no changes in the diameter of muscle fibers due to the change in the dietary protein source. It is suggested that studies be continued, evaluating the effect of different diets on animals undergoing strength training, in addition to collecting data on liver markers and evaluating pH, citrate and nitrogen in urine.