Perfil bioquímico de amostras de aborto infectadas por parvovírus e circovírus suíno por espectroscopia Raman

Abstract

Reproductive failure in swine farms is a problem for production due to the loss of animals and the associated economic loss. Porcine parvovirus (PPV) is one of the main infectious causes of loss of reproduction, especially when co-infected with porcine circovirus (PCV2). The detection tools used and already protected so far are quite sensitive and specific, however, they have a high associated value and time to obtain the results. Easy-to-handle and innovative diagnostic strategies such as the proposal, when defensive, could favor large-scale, rapid and on-the-spot diagnosis. The present work evaluated possible molecular biomarkers of infection by PCV2 and PVV through Raman spectroscopy. Identifying for circovirus infection biomarkers for the intensities of peaks at 1604 cm-1 and 1670 cm-1, for parvovirus infection intensities for the peaks at 675 cm-1, 744 cm-1, 1240 cm-1, 1310 cm-1 and 1340 cm-1 and for both communities the intensity of peak 704 cm-1. The results obtained for the established database supply, making the technique promising for viral diagnosis.