Validação do método de análise de Salmonella via detecção molecular em gelatina

Abstract

Salmonella is a serious problem when it comes to food products of animal origin, and can lead to the death of infected patients. This study aims to evaluate the efficacy and reliability of the 3M® MDS commercial solution in detecting Salmonella in samples of bovine gelatin, comparing it with the ISO 6579-1 reference methodology, since the operating mechanism of this solution can generate erroneous results due to the presence of DNA from dead cells. By comparing the results of determination of 120 gelatin samples with the ISO 6579-1 methodology, it was found that the MDS presented 100% efficiency, with no false-positive or false-negative results. The analysis time of the methods, the economic aspects and the requirements of each analysis were also compared, aiming at a scenario of increasing the number of daily analyses. It was concluded that the MDS protocol has a shorter time to release results, better scalability and a higher cost of inputs per analysis.