Diversidade de vírus específicos de insetos identificados por sequenciamento de nova geração em culicídeos no Brasil: revisão sistemática de literatura

Abstract

Insects harbor a diversity of viruses, including viral species that affect public and animal health (arboviruses) and insect-specific viruses (ISV). The latter are viruses restricted to insects but can be useful for the biological control of arboviruses and insect pests. Accessibility to Next Generation Sequencing (NGS) technologies has enabled the discovery of a wide variety of ISVs, which would not be possible using classical methods for viral detection. In this context, the present literature review carried out a survey of the diversity of ISVs identified in mosquitoes (Diptera: Culicidae) in Brazil using NGS technologies. The search for studies was carried out from the Web of Science (WoS) collection using the following combination of descriptors: “Virome” OR Virom* OR “Virus Discovery” AND “Insect Viruses” OR “Insect Specific Virus” OR “Mosquito Viruses” OR “Mosquito Specific Virus” AND “Culicidae” OR Culicid* OR “Mosquito” OR Mosquit*. The documents obtained were filtered using the following filters: original article, English language, open access, Brazil and term “virus”. 18 publications were used in this review, in which 56 species of ISVs were identified, 43 of which were new records. Most of the ISVs identified had a single-stranded RNA genome, associated with the Flaviviridae family and Negevirus taxon, and were predominant in mosquitoes of the genera Aedes and Culex. Illumina Sequencing technologies were the most used and were mostly used to describe the virome of mosquitoes collected in the Northern region of Brazil. These findings show that the application of NGS to identify ISVs circulating in mosquitoes in Brazil has provided the description of many previously unknown viruses, which contributes with new information regarding the composition of the virosphere.