Estabelecimento e regeneração in vitro de embriões maduros de Phaseolus vulgaris

Abstract

The common bean (Phaseolus vulgaris) is one of the main legumes, both in agriculture and for food security. Due to the high demand for consumption of this legume, genetic improvement programs seek to develop new varieties that stand out in the field, bringing high productivity and resistance to pests, diseases and other various stresses that can occur in the field. One of the tools for carrying out genetic improvement is in vitro cultivation, however, this technique encounters many difficulties in establishing this culture in in vitro. Therefore, the objective of this work was to establish and regenerate mature embryos using two culture media, B5 and MS, testing which of the media promotes better development for the SCS Querência cultivar. In an attempt to establish the embryos, the phytoregulators BAP (6-Benzylaminopurine) with concentration 0,3 mg L −1 and AIB (Indolebutyric Acid), with concentration 0,1 mg L −1, were added to the culture media. The data were analyzed with the help of Excel using mean and standard deviation. The results obtained demonstrated that the B5 culture medium supplemented with 0,3 mg L −1 of BAP and 0,1 mg L −1 of AIB is more efficient to regenerate zygotic embryos of the cultivar SCS Querência, where 52% of the embryos managed to regenerate and develop leaves, however the concentration of 0,1 mg L−1 of IBA was not enough to form roots.