Avaliação de protozoários ruminais de búfalos alimentados com azevem

Abstract

The objective of this work was the quantification of protozoa in the ruminal fluid of buffaloes fed with ryegrass. The animals were slaughtered at the Orfimar slaughterhouse in the municipality of Itapejara do Oeste - Paraná, the liquid collection was carried out shortly after the animal was slaughtered, according to analyzes carried out in the microbiology laboratory of the Federal Technological University of Paraná, Campus Dois Vizinhos. The ruminal fluid of the buffaloes was collected and then the pH was measured using an electronic meter. The sample was stored in plastic bottles with 200 mL of ruminal fluid and 200 mL of 18.5% formalin, then 1 mL of ruminal fluid and 9 mL of 30% glycerin were placed, adding three drops of lugol in test tubes, after After 15 minutes, 1mL of this liquid was placed in the adapted Sedgewick-Rafter chamber. The number of protozoa/mL of ruminal fluid was relatively high, with an average of 158.4 x104 due to the high fiber content in the diet, causing a high rate of salivation, and the optimal ruminal pH, influenced by salivation.