Espectroscopia Raman/SERS aplicada ao monitoramento de antibióticos

Abstract

Rapid and accurate detection of antibiotics is a critical challenge in healthcare, but a new approach based on Raman/SERS spectroscopy may be a solution. This research presents the Raman spectrum acquisition (“fingerprints”) of seven different antibiotics using this technique. The antibiotics evaluated were ampicillin, meropenem, ceftazidime, polymyxin B, vancomycin, gentamicin, and ciprofloxacin. Four excitation wavelengths were used to obtain the Raman spectra, namely 532 nm, 633 nm, 785 nm, and 1064 nm. In order to amplify the Raman signal of the antibiotic when diluted in water, nanoparticles were synthesized by the LASiS (Laser Ablation in Liquid Solution) technique and used as mediators of signal amplification. The Nps were characterized by various techniques, including TEM, AFM, DLS, UV-Vis, and zeta potential. The analyses allowed a better understanding of the properties of the nanoparticles and how they can influence the SERS spectrum acquisition of each antibiotic. The results showed that the Raman spectroscopy technique could provide unique spectra for each type of antibiotic evaluated, allowing for precise identification. Furthermore, the nanoparticles synthesized by the LASiS technique can improve the sensitivity and reproducibility of the Raman spectroscopy technique. It was possible to obtain SERS spectra of antibiotics using Au, Ag, Co, Cu and V Nps. The data obtained in this study suggest that the Raman spectroscopy technique is a promising option for identifying different types of antibiotics in samples of different origins.