Desenvolvimento de um dispositivo de baixo custo para a análise da Concentração Inibitória Mínima (CIM)

Abstract

The Minimum Inhibitory Concentration (MIC) assays determine the lowest concentration of an antimicrobial agent that prevents visible growth of a microorganism. Despite the countless technological advances in the field of electronic laboratory instrumentation equipment, the colorimetric methods for determining MIC are performed visually, without any instrumentation equipment. This means that the analysis depends on the analyst’s perception, becoming subjective and compromising the analysis, since the human eye is unable to perceive small nuances in the countless shades of colors. In this way, the objective of this work is to develop a low-cost instrumentation device, which will perform the acquisition of digital images in a controlled environment to later perform the processing of these images, in an attempt to accurately determine the value of the Inhibitory Concentration Minimum.