Extração de corantes antioxidantes da casca do pinhão (Araucaria angustifolia (Bertol.) Kuntze)

Abstract

Araucaria angustifolia is a species found in South America and produces pine seeds. According to some existing research, there is the presence of phenolic compounds and antioxidant activity in the pine shell, material that is discarded after consumption. In an attempt to avoid wasting this material, this research aimed to optimize the extraction process of phenolic compounds present in the pine shells. From the experimental design, in which the ethanol concentration and extraction timewere varied, the best condition to extract the compounds was obtained, being 50% ethanol (v v-1) and 10 minutes in ultrasound for total phenolic content , ABTS and FRAP, while for DPPH, the ideal condition was 20% ethanol (v v-1) and 5 or 10 minutes of extraction. The content of phenolic compounds in the extract was 75,30 - 124,17 mg EAG g-1. The antioxidant activity had the following values 180,15 - 196,52 μmol of Trolox g-1 for the kidnapping method of DPPH radical, 7127,19 - 17387,22 mmol L-1 of Trolox g-1 for the kidnapping method of ABTS radical and 10787,44 - 14109,76 mmol L-1 of Fe2+ g-1 the methodology for the reduction of iron (FRAP). The results show that for some plants, the pine seeds has a high content of phenolic compounds and high antioxidant activity.