Modificação química de celulose bacteriana visando curativo antimicrobiano

Abstract

Skin lesions from burns require attention in the treatment to avoid infections and significant complications during the healing process. An important characteristic to consider in the proposed dressings material is providing a moist and warm environment to improve healing. Bacterial cellulose is presented as ideal for this application due to its biocompatibility, high crystallinity, water absorption capability, and thermal stability. Although it has favorable characteristics, the material does not have antimicrobial properties. Therefore, we propose a modified bacterial cellulose film to have antimicrobial properties. The modification is proposed for the dressing to be able to adsorb an antibiotic and release it in the aimed site. This property was gained through the esterification reaction of 6-pyridinehexanoic acid and the cellulose film to retain an anionic drug (oxacillin). Besides, it used anionic liposomes, encapsulating the drug, and adsorbed to the cellulosic film for slower release. The pyridinium salt molecule was synthesized and connected to the bacterial cellulose, followed by adsorbing the drug. The modified film could adsorb 14,2% of the drug present in the medium, 6,8 1% related to its mass. The liposomes presented 20,1 % of encapsulation efficiency. Thus, we also produced a BC film with liposomes that could retain 16,5 % of oxacillin related to the cellulose mass. Meanwhile, the first film released half of the drug adsorbed in 12 hours, the film with liposomes took four days to start releasing and kept releasing for other 11 days. The produced films were analyzed by infrared (FTIR), nuclear magnetic resonance (NMR), UV-Vis spectroscopy, high-performance liquid chromatography (HPLC), and scanning electronic microscope (SEM).