Efeito de fertilizante orgânico a base de extrato pirolenhoso em testes de toxidade aguda e crônica sobre Folsomia candida

Abstract

Ecotoxicologyis defined as a science that studies how substances act on living organisms. It is essential in defining non-lethal doses of contaminants, especially when it comes to compounds that are identified in soil and water. Within this context, the present study aimed to evaluate, through ecotoxicological assay with Folsomia candida, an acute and chronic toxicity of different doses of organic fertilizer based on pyroligneous extract. The methodology adopted is by Oliveira Filho et al. (2018), which followed the principles as normative ISO -International Organization for Standardization for Collembola. Data were obtained through analysis of variance (One-way ANOVA) followed by Dunnett's test of means (p <0.05). The priolenous layer used commercially is Biopirol®, produced by the company Biocarbo Ind. Com. Ltda. The test evaluated two variables for 28 days on Folsomia candida: acute toxicity (appear) and chronic toxicity (reproduction). The design was randomized, with 8 treatments in the recommendations of 0; 0.05; 0.1; 0.2; 0.5; 1; two; 3%, in 6 repetitions. For acute toxicity, there was a statistical difference in the treatments D0.2 (dose 0.2 ml, concentration 0.1%) and D4 (dose 4 ml, concentration 2%), it is not possible to calculate the lethal dose that decreased50% of the population do the test (LC50). As for the chronic toxicity test, from the first dose tested it showed a difference, the dose that reduces 50% of the reproductive capacity of the collemboli was EC50 = 25.15 ml. The results found indicate that the doses of pyroligneous extract are capable of affecting the reproduction and occurrences of Folsomia candida, and this work is a basis for further research involving ecotoxicological studies and Biopirol® as a soil fertilizer.