Extratos orgânicos das folhas de Eugenia involucrata DC e atividade antimicrobiana

Abstract

Eugenia involucrata DC belongs to the Myrtacea family, popularly known in Brazil as cerejeira, cerejeira do mato, cerejeira do rio grande. It is found predominantly in the South, such as Paraná, Santa Catarina and Rio Grande do Sul, but also has occurrences in other states such as São Paulo, Rio de Janeiro, Minas Gerais and Espírito Santo. This plant has some properties, such as anti•inflammatory, antioxidant, antimicrobial, antidiarrheal, among others. The objective of this work was to obtain extracts from the leaves of E. involucrata, using solvents in polarity gradient, petroleum ether, dichloromethane, ethanol and distilled water, until the plant was exhausted, and thus test these extracts against Gram bacteria. •positive S. aureus and B subtilis, Gram•negative S. enterica Typhimurium and E. coli, by disk diffusion methodology and after determining the minimum inhibitory concentration. The extracts showed the following yields: petroleum ether 2.66%, dichloromethane 5.61%, ethanol 12.01% and distilled water 8.24%. The disk diffusion tests showed inhibition halos for the ethanol and water extracts against the four bacteria tested, the petroleum ether and dichloromethane extracts did not show inhibition halos. The minimum inhibitory concentration (MIC), ethanol for E. coli showed good (MIC <100 μg mL•1) and moderate (MIC 500•100 μg mL•1) activity for S. aureus and B subtilis S. enterica Typhimurium. Water extract showed moderate activity against the four microbial strains (MIC 500•100 μg mL•1).