Filmes de celulose bacteriana modificados como curativos: filmes lipofílicos e doadores de oxido nítrico

Abstract

Bacterial cellulose is considered a chemically well-recognized material of interest to the biomedical field. In its pure form it can undergo modifications that can give it properties for all the essential requirements to function as a dressing material. Its high porosity and surface area allow a high potential for the introduction and release of antimicrobial agents, drugs and other biofunctional materials. In the investigation of new types of biocuratives, the inclusion of antimicrobial characteristics is extremely important, since it can considerably improve the efficiency of the material in relation to treatments of chronic wounds or susceptible to microbial infection. Some antimicrobial characteristics little explored in dressings are the use of hydrophobic layers and the presence of nitric oxide (NO), which has a series of effects on cells and also on bacteria. In this way, in this work we aimed to functionalize the cellulose membrane, modifying and characterizing films in order to improve its applications and wound restorations by improving its antimicrobial and biocompatibility activity. For this purpose, conjugate hydrophobic molecules on its surface and additionally to prepare films capable of donating nitric oxide (NO) were proposed. Organic synthesis reactions were performed to prepare lipophilic organic compounds and trimethylammonium cation derivative for coupling on bacterial cellulose films. Different samples of the bacterial cellulose film were characterized from measurements of contact angle, absorption spectroscopy in the infrared region, nuclear magnetic resonance, fibroblast growth test and antimicrobial test. Comparatively, membranes modified with hydrophobic substances show greater cytocompatibility with fibroblasts when their hydrophilic / hydrophobic balance was decreased. In addition, Roussin's black salt was synthesized and coupled to the bacterial cellulose membrane. For the coupling of Roussin's black salt, the membrane that has no modification showed no effective interaction with the salt solution. Considering that the modification of the membrane and its hydrophobicity effect brought positive characteristics to these films, lipophilic films showed an improvement in antimicrobial activity and in cytocompatibility compared to unmodified cellulose. Films containing nitric oxide donors have been prepared, characterized and will be analyzed for their ability to release nitric oxide.