Avaliação no teor de hidrogênio no biogás produzido a partir de manipueira com adição de glicerol residual por biodigestão anaeróbica

Abstract

In recent years the production of liquid and gaseous biofuels, biogas, a mixture of methane and hydrogen, have been increasing significantly as a function of increased energy demand, in addition to the growing need for treatment of waste agribusiness. The present study aimed to evaluate the production and composition of biogas by gas chromatography, perform characterization of substrates used, manipueira and glycerol, by UV-VIS Spectrophotometric method, and microbiological characterization of inoculum, through the isolation of microorganisms, DNA extraction, PCR, electrophoresis and genetic sequencing. Four different anaerobic microorganisms were found in the inoculum, three belonging to the Genus Bacillus and a one to the genus Brevundimonas. The use of glycerol as additive the manipueira proved to be feasible, since the treatments containing this substrate were obtained hydrogen volumes far superior compared to the treatment that contained only manipueira. A second model was proposed to describe the production of hydrogen by biodigestion process, the model described how suitable experimental data, showing value of R2 of 0.98. From the model, it was estimated the maximum volume of hydrogen 1063.4 mL, with the addition of 3.38% (v/v) glycerol. In this way, the addition of glycerol to manipueira for the anaerobic biodigestion process proved to be viable, since they were obtained in increments biogas production to the tune of 4 times when compared to treatment without this additive.