Bioprospecção de fungos produtores de enzimas celulolíticas na furna do Passo do Pupo 1

Abstract

In the dynamic landscape of global technological development, has that contemporary processes are redesigned every day, where new practices are added or replaced by more economical and better operating conditions options in this context the study about the action and the search for new sources of enzymes, such as cellulase, which play an important role in this process by improving the property of degradative cellulose, can be used in different industrial segments. This work aims to isolate strains filaments fungi able to hydrolyze cellulose from a bioprospecting in Furna do Passo do Pupo 1, located in Ponta Grossa - PR. Using the culture medium composed of Manachini solution (KH2PO4, 2g.L-1; (NH4) 2 SO4, 1-1g.L; MgSO4.7H2O, 0,1g.L-1; Na2HPO4.2H2O, 0 , 9g.L-1, yeast extract, 1g.L-1), supplemented with carboxymethylcellulose (CMCA) 0.5% (w / v), there was a qualitative selection of the best strains to total 7 isolated fungal colonies. These had enzyme activities (IE) and growth rate (CT) determined in the course of 5 days of incubation at 20 ° C with no light, from the disclosure of daily hydrolysis halos with solution of Congo 0,1% (w / v) measured for the calculation of the IE and CT data by the ratio between the diameter of the halo (mm) by the well diameter (mm) and because of halo diameter (mm) with the incubation time (day) respectively. All the strains were characterized with good as fungi cellulase producing capability, since all showed SI ≥ 2.0, 4 strains (G0 (2), G0 (5), BE1 (9) and DE2 (10)) showed values below 6.0 IE ranging from 3.6 to 5.78, and 7 strains (CE2 (1), DE1 (3), G0 (4), DD2 (6), DE2 (7) G0 ( 8) and BE1 (11)) presented ≥ IE 6.0, IE ranging from 6.16 to 7.17, results that fall as excellent producing cellulase, noting that DE2 strain (7), with higher IE, presented the culmination of enzymatic growth rate of approximately 36 hours, standing out from the others presenting the apex of Tc from 44 hours of incubation, the fact of utmost importance for the industry, which focuses on the search for processes ever more efficient. The results show the potential of the isolated strains, thus expanding the need to identify individuals found, due to the high possibility of these being endemic of the rated site.