Produção de single-cell-oil em batelada alimentada

Abstract

Biotechnological compounds derived from microorganisms are increasingly presenting themselves as an alternative to conventional forms of production to obtain products of interest. Several microorganisms have been studied in this sense, because they can be a constant and renewable source of resources that do not suffer climatic influences for their production. The yeast Rhodosporidium toluroides has presented itself as an important source of microbial oil due to its great capacity to accumulate intracellular lipids, being considered an oleaginous microorganism. The production and accumulation of lipids by yeast Rhodosporidium toluroides requires that the culture be performed in a medium with excess carbon, so that when the cellular multiplication of the yeast ceases, this carbon is converted into energy reserve in the form of intracellular lipids (single-cell-oil, SCO). The production of SCO by the yeast Rhodosporidium toluroides can be performed in several ways and strategies. The objective of this work was to address the production of SCO through a batch strategy fed into a bioreactor using urea and sucrose as a source of nitrogen and carbon, respectively. Optimization of urea and sucrose concentrations in the culture medium was performed in order to increase biomass productivity and intracellular lipid accumulation. The efficacy of the fed batch strategy adopted using three feeds during the bioreactor period was also verified. Fermentation in a bioreactor after 144 hours using 3 intermittent feeds obtained 35.85g/L of biomass being accumulated 17.87% in intracellular lipids.