Estudo da interação de lipossomas furtivos com anestésico local ropivacaína

Abstract

The controlled and specific drugs delivery is a important development in the treatment of various diseases. In turn, doses given are smaller and targeted to specific treatment and analgesia sites. These advantages reduce the toxicity of the drug in the body. Accordingly, the controlled drug delivery area must be continuously exploited for the development of formulations with increasing degrees of effectiveness. In this sense, the use of carriers is of great importance for the drug release benefits to be achieved. Local anesthetic encapsulation (ALs), such as ropivacaine (RVC), has already been studied in normal liposomal systems, however, the literature brings little researches about the behavior towards furtives liposomes (FL). The main objective of this work is study of the building and interactions of the RVC/LF complex using advanced nuclear magnetic resonance techniques (NMR-STD and DOSY). The low saturation transfer values from LF hidrogens to RVC hydrogens confirm the encapsulation of the LA in the LF vesicles and corroborate with the low values of the complex fraction and association constant obtained in the DOSY experiment. However, the STD spectrum indicates that the entire RVC molecule was encapsulated, since aromatic hydrogens and the piperidine cycle underwent saturation transfer, observed through the normalized STD map.