Produção de extratos dos resíduos de indústria vinícola e sua avaliação antioxidante e citotóxica

Abstract

Cancer is one of the biggest health problems worldwide and due to the increase of tumors and the side effects of traditional medicines, it is necessary to recognize new antitumor substances, especially the natural ones. The grape (Vitis labrusca L.) has several compounds that are beneficial to human health and is consumed in different ways, especially in the form of whole juices. Thus, considering the great generation of grape residues during the production of its juices, and that this bagasse still has many bioactive compounds present in the fruit, it was sought to obtain extracts of the organic grape marc for application in the pharmaceutical industry, aiming the production of medicament with antitumor activity. The extracts were produced using the solvents ethanol and ethyl acetate by the immersion method at 35 ° C, the extraction yield using a S / F (solvent / mass) = 75, 9% (ethanol) and 5% (ethyl acetate). The antioxidant potential of the extracts was performed using Trolox®-DPPH and showed a concentration of 196.5 μM TEAC / 100g ethanolic extract and 178.8 μM TEAC / 100g extract with ethyl acetate. The cytotoxic effects of the extracts were evaluated by the MTT test with culture of rattus norvegicus (HTC) hepatoma cells, exposed at 24, 48 and 72 hours at concentrations of 50, 75, 100, 150, 200, 250, 500, 750, 1000 and 2500 μg of the extract / ml culture medium. The concentrations tested at the time of 24 hours, for both extracts, did not present cytotoxicity to the tumor cells. At the 48 hour time, the concentrations of both extracts provided an increase in cell viability, i.e., caused the proliferation of the HTC cells. At the time of 72 hours, the concentrations of 50, 75 and 100 μg / mL of the extract obtained with the ethyl acetate solvent and all the concentrations tested with the ethanolic extract were cytotoxic. In general, the ethanolic extract presented higher amount of antioxidant compounds and better antitumor activity. Thus, the data of the present study propose a use of residues of the wine industry in the pharmaceutical industry, for the production of antitumor drugs.