Produção de biossurfactantes por Bacillus subtilis

Abstract

Some microbial species produce biosurfactants and these have advantages when compared with surfactants chemicals mainly because they are not toxic and biodegradable . Why be a product from microbial metabolism, the production of biosurfactant is related to the factors influencing the microbial growth, among them are the sources of carbon and nitrogen as well as the ways of conducting the fermentation process. Using the bacterium Bacillus subtilis , the aim of this study was to verify the production of biosurfactant in culture media with varying amounts of nutrients, and determine the content of hydrocarbon emulsion (Hexane and Carbon tetrachloride). The emulsifying inde x was determined by the height of the emulsion formed, being your result expressed in percentage. According the factorial experimental planning 2 (6-2), and based on experimental results, the ideal conditions for a high percentage of emulsification of hexan e, were highlighted in the experiment 06, constituted of the base medium with 2.0% of soybean oil, 2.0% of corn oil, 20.0% of glycerol, 1.0% glucose, 0.1% ammonium sulfate, 2.0% yeast extract, with an index of 23.30% emulsion. Analyzing the results obtaine d through the emulsifying index of carbon tetrachloride best evidence were seen in the experiment 03 using base medium plus 0.2% soybean oil, 20.0% corn oil, 2.0% glycerol, 1.0% glucose, 1.0% ammonium sulfate, 2.0% yeast extract, obtaining an emulsion of 6 2.20%, both growth was by bacteriological growth for 48 hours at 30°C and incubated in shaking table at 200 rpm and with temperature control at 30°C for 144 hours . Thus, the results presented show that the bacterium Bacillus subtilis demonstrated poten tial for the production of biosurfactant.