Atividade de enzimas proteolíticas de diferentes espécies microbianas

Abstract

Considering the increasing use of technologies that minimize the environmental impacts caused by toxic and polluting compounds, the use of proteases in large scale appears as an alternative to the conventional chemical processes applied in current industrial processes. The degradation of industrial waste, with polluting capacity, can be carried out by biotechnological processes, using microbial enzymes linked to the process of degradation of the pollutant. Thus, this work aimed to determine the proteolytic enzymatic activity of Bacillus subtilis and Pseudomonas aeruginosa. The experiment was carried out at the Laboratory of Microbiology of the Federal Technological University of Paraná, Francisco Beltrão Câmpus. The qualitative determination of the enzymatic activity of B. subtilis and P. aeruginosa was performed in triplicate by the method of stinging in solid culture media, called Milk Agar, containing 18 g.L-1 of agar, 2.5 g.L-1 of yeast extract, 1 g.L -1 of glucose, 2.5 g.L-1 of NaCl and 100 mL.L-1 of the induction substrate, in this case, skim milk. The enzymatic activity was determined through the relationship between the mean halo diameter and the mean colony diameter, expressed as Enzymatic Index (EI) and for statistical analysis, the Student's t-test was used. Based on the experimental results, the qualitative bioassay of the enzymatic activity of Bacillus subtilis and Pseudomonas aeruginosa presented a positive result for the production of proteases, and there was no significant difference between the means of EI (p≥0.05).