Padronização de bioensaio para monitoramento de Microcystis aeruginosa toxigênica

Abstract

Cyanobacteria are found in aquatic environments and their blooms occur as a result of eutrophication caused by anthropogenic activities. The Microcystis aeruginosa species is a cyanobacteria widely found in water bodies and producing microcystin able to promote poisoning and liver tumors. The monitoring cyanotoxins include chromatographic analysis, spectrophotometric, immunoassays, among others. Due to high costs, these analyzes are not available in all quality control laboratories of water for public supply. The objective of this work is to standardize bioassay Artemia salina aimed at application in the monitoring of Microcystis aeruginosa toxigenic due to ease of implementation and low cost. The bioassay Artemia salina was standardized, considering the cellular concentration of Microcystis aeruginosa (BCCUSP232 strain) of the following levels of monitoring: up to 10.000 cell.mL-1 (Supervisory Level), 10.000-20.000 cell.mL-1 (Level Alert 1) 20.000-100.000 cell.mL-1 (Level Alert 2) and greater than 100.000 cell.mL-1 (Level Alert 3), recommended by the WHO. Was performed in different proportions: 2,0 mL of test sample (100%), 1,0 mL of the test sample (50%), 0,5 mL of the test sample (25%), 0,25 mL of the test sample (12,5%), 0,124 mL of the test sample (6,2%) and 0,062 mL of test sample (3,1%). The indication of toxicological is the proportions of the test sample between 12.5% and 25%, where the Artemia salina showed a better response considering a viable alternative for the quality control of water for public supply.