Avaliação da cepa de Lactobacillus plantarum LPB-R01 como inoculante na silagem de cana-de-açúcar

Abstract

Sugarcane silage is a bulky alternative that has been gaining ground without a Brazilian scenario, due to its high productivity per area.However, this is a forage that has great losses of dry matter and energy in its fermentation process, losses that can be minimized with the use of inoculants. The objective of this study was to evaluate the potential of the Lactobacillus plantarum strain LPB R-01 as inoculant in the fermentation process of sugarcane silage. The experiment was developed at the Universidade Tecnológica Federal do Paraná, not Câmpus Dois Vizinhos, being a silage company by a rural producer of Boa Esperança do Iguaçu, with a harvest and a fully mechanized machinery with a piece size of around 3 cm. The strain was created by the group of biotechnological processes of UFPR-Curitiba. There were 72 samples, arranged in two treatments, one with the inoculation of the bacteria in the concentration 106 CFU / g of silage and another one was the control, being three repetitions. For the preparation of the silos were used transparent plastic bags of 12 microns, which were placed in a four liter beaker and with a wooden stick compacted the samples, to establish an anaerobic environment. Each sample had a weight around one pound and forty grams. At the end of the fermentation time of each sample, they were frozen until the analyzes were carried out. For the analyzes of pH, titratable acidity, ammoniacal nitrogen and reducing sugars, the frozen samples were used in the fermentation process of 0, 12, 24, 36, 48, 60 and 72 hours and for the bromatological analyzes samples of 0, 7, 15, 30 and 45 days. The results obtained did not show significant effects on the inoculation of the strain in sugarcane silage. Only the levels of FDA showed interaction between treatment and day, the other analyzes only differences between days. The results of MS, PB, NDF, FDA, HEM, MM, LIG and Nammoniacal, range from 27.26% to 21.72, 3.11% to 4.2%, 76.03% to 54.89 %, 10.9% to 29.47%, 3.91% to 5.54%, 7.06% to 9.63% and 1.8% to 1.32% respectively. However, the pH values presented a constant decrease and the final value with the inoculant was 4.41 and without the same of 4.54; the acidity levels consequently increased constantly and the sugars there was a great variation. This work concludes that the LPB R-01 strain of Lactobacillus plantarum showed significant results of the interaction treatment and day for the analysis of FDA, but only with this significant result between the treatment with the inoculation of the bacterium and the control we can not conclude that the strain is efficient in the fermentation process of sugarcane silage.