Armazenamento e teste de tetrazólio de sementes de jabuticabeira

Abstract

The jabuticaba (Plinia sp.) Is an important crop native to our country, which has its biggest potential market marketing fresh and processed. Presents as the main form of multiplication through seminiferous, even if they present characteristic recalcitrant. The fact that enables this form of propagation is by polyembryony be. As an alternative to the conservation of recalcitrant seeds are techniques of using biofilm and vacuum. The aim of this study was to evaluate the vacuum packing and biofilms in the viability seeds of jabuticaba tree [Plinia trunciflora (O. Berg) Kausel], as well as, verify if the tetrazolium "test" can be applied in these seeds. The experiment was carried out at the Plant Physiology Laboratory and in the Nursery at the Universidade Tecnológica Federal do Paraná - Câmpus Dois Vizinhos, State of Paraná, Brazil. The experimental design was randomized blocks, in factorial 2 (vacuum use) x 3 (coating type) x 5 (storage time), with four replication of fifty seed by plot. The seeds were extracted and it was separate in two lots, one to use for the vacuum packing and the other not. Each lot was separated in three sub-lots according to the coating type [ cassava starch (3% m v-1), chitosan (3% m v-1) and without biofilm]. Then, the seeds were stored in cold chamber using constant temperature of 5 ± 1°C for 7, 14, 21, 28 and 35 days. The emergence percentage, seed viability by the tetrazolium "test" and emergence speed index were evaluated. It was recommended to store the jabuticaba seeds in vacuum packing. If it is not possible to use de vacuum packing, the jabuticaba seeds should be coated with biofilm-based chitosan or cassava starch. The tetrazolium "test" proved to be feasible and faster to evaluate the jabuticaba seeds viability.