Potencial da ação antifúngica do licor pirolenhoso de Hovenia dulcis Thunb. a fungos xilófagos in vitro

Abstract

The present work was be carried out in the municipality of Dois Vizinhos, in the Wood of Technology Laboratory and in the Phytosanitary Laboratory of the Universidade Tecnológica Federal do Paraná, Dois Vizinhos-PR campus, with the objective of analyzing the potential of the pyroligneous liquor of Hovenia dulcis as a natural inhibitor in culture media inoculated with the fungi Trametes versicolor (white rot) and Neolentinus lepideus (brown rot). The treatments used in the experiment were: 0% (T1 - witness), 0.5% (T2), 1% (T3), 2.5% (T4), 5% (T5) and 10% (T6) concentration of pyroligneous liquor in BDA. The fungi of Trametes versicolor and Neolentinus lepideus were inoculated in the semi solids culture media, by means of mycelium discs of the fungi already in pure colony, and then taken to BOD, for 13 days to grow the colonies. For each of the treatments, 8 petri dishes (replicates) inoculated with the fungi in BDA culture medium were used. At concentrations ≤ 0.5% the pyrolignous liqueur made the development of the white rot fungus difficult. However, in concentrations > 0.5% the liquor presented inhibitory action to fungus growth. Regarding brown rot fungus, it was found that only on the witness plates (0% liquor concentration) did the fungus develop. In none of the other five treatments did the fungus develop. This proves the inhibitor effect of pyroligneous liquor on fungus control. Based on the methodology employed in vitro and the results obtained, it is concluded that the pyrolignous liquor of the Hovenia dulcis wood has a great inhibitory action. The same proved to be efficient when submitted to treatment in wood fungi (white rot - Trametes versicolor and brown rot - Neolentinus lepideus). Future experiments with concentrations 0,1% to 1% are recommended to assess the minimal concentration of the product that inhibits fungal growth.