Sistema embarcado e programa de controle para um microscópio In Situ

Abstract

This document describes the project, the design, the implementation and the tests of an In Situ Microscope used for monitoring microorganisms in a bioreactor. The project consists of an optical system with a 40x lens, a CCD (Charge-Coupled Device) camera to image acquisition, a lighting system of the microorganisms within the bioreactor and a PC for monitoring and processing images. The system allows the user to choose which desired lighting. There are two different light sources, LED (Light-Emitting Diode) and SLD (Super Luminescent Diode). This illumination is accomplished with pulses of short duration, which can be set between 100ms and 500ns. Monitoring of microorganisms is achieved through a window of visualization in the bioreactor. In the window is an optical system coupled with a 40x lens, it has a CCD camera. The camera captures images and transmits them to the PC via a Firewire interface (IEE1394). Through the microcomputer the microorganisms are monitored online in the bioreactor. In addition, you can perform a treatment of the images and determine, for example, the concentration of microorganisms or analyze the morphology of animal cells.